AB013. Retroperitoneal laparoscopic surgery for the upper urinary tract: the Institute of Urology, Peking University (IUPU) technique

immunofluorescence of bladder smooth muscles of OAB rats were shown. The positive staining of TRPC6 appeared at cell membrane of bladder smooth muscle. The western blot and RT-PCR results indicated that, the expression of TRPC6 protein and mRNA in OAB was significantly higher than the normal. After administration of FK506, the expression and mRNA of TRPC6 decreased obviously, and there were improved contraction of smooth muscle in OAB rats. TRPC6 protein could be elevated after induction of PDGF in BMSCs. And blockade with the inhibitors of JNK and P38, the protein levels of TRPC6 were decreased. Conclusions: The expression of TRPC6 protein and mRNA in bladder smooth muscle of OAB was significantly higher than normal. Reduced the expression of TRPC6 can improve bladder function and contraction of smooth muscle in OAB. PDGF could induced TRPC6 expression in BMSCs. P38 and JNK were involved in the regulation of TRPC6 expression in BMSCs.